ac recombinant human vegf protein r d systems  (R&D Systems)

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex-specific lymphatic responses to estrogen shape atherosclerosis in high-risk mice

doi: 10.3389/fcvm.2026.1699372

Figure Lengend Snippet: VEGF-C 152s treatment prior to ovariectomy limited the negative effects of subsequent E2 supplementation on lymphatic function and plaque burden in ldlr −/− mice. (A) Experimental design. 7 weeks old Ldlr −/− female mice received IP injections of VEGF-C 152s (e.g., VEGF-C) or vehicle. At 11 weeks, mice underwent either bilateral ovariectomy (OVX) or sham surgery, and switched on a high-fat diet (HFD). Estrogen (E2) supplementation began one week later. Lymphatic function was assessed in mice immediately prior to sacrifice at 20.5 weeks, after which organs were harvested. Green, orange and pink dashed lines represent VEGF-C 152s injections, HFD, and E2 implant durations, respectively. (B) Total body weight at the time of sacrifice and (C) over 21 weeks were reported. (D) Weight of visceral adipose tissue and (E) weight of the uterus, each expressed as percentage of total body weight. (F) Plasma total cholesterol and, (G) lipoprotein profile were assessed. (H) Contraction frequency of the popliteal collecting lymphatic vessels was measured by intravital microscopy. (I) Representative images of en face oil-red-O staining of the aorta and quantification of atherosclerotic plaque burden, expressed as percentage of total aortic area. Data points represent individual animals. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. HFD, high-fat diet; OVX, ovariectomy; VEGF-C, vascular endothelial growth factor C; CM, chylomicron; VLDL, very-low density lipoprotein; LDL, low-density lipoprotein; HDL, high-density lipoprotein.

Article Snippet: Mice received intraperitoneal (IP) injections of VEGF-C (152s) at a dose of 50 ng per 25 g of body weight [purified recombinant rat VEGF-C protein (152s), Fitzgerald, catalog no. 30R-AV006-22] or vehicle control (phosphate-buffered saline, PBS), administered three times per week for four consecutive weeks.

Techniques: Clinical Proteomics, Intravital Microscopy, Staining

Journal: Exploration of BioMat-X

Article Title: Top2b regulates morphological and migratory properties of retinal progenitor cells in vivo and upon transplantable matrix substrates

doi: 10.37349/ebmx.2025.101335

Figure Lengend Snippet: Figure 6. Cultured retinal progenitor cell groups with Top2b overexpression (OE) and knockdown (KD) illustrate altered expression of select chemotactic receptors compared to wildtype cell groups with no Top2b manipulation (WT). Cell groups illustrated significant differences in the expression of selected chemotactic receptors: vascular endothelial growth factor receptor (VEGFR1), fibroblast growth factor receptor (FGFR1), and c-x-c chemokine receptor 4 (CXCR4, receptor for the stromal cell-derived factor 1, SDF-1a, ligand). Statistical significance across the cell groups is denoted by p-value: * < 0.05, ** < 0.01, **** < 0.0001 as via two-way ANOVA tests followed by Dunnett’s post-hoc test

Article Snippet: Approximately 600 μL of each growth factor vascular endothelial growth factor (VEGF; R&D Systems, Minneapolis, MN, USA, 564-RV), fibroblast growth factor-8 (FGF-8; Invitrogen, Carlsbad, CA, USA, PHG0184), and stromal derived factor 1-a (SDF-1a; Sigma Aldrich, St. Louis, MO, USA, SRP3276) was added to the bottom of separate transwell assays.

Techniques: Cell Culture, Over Expression, Knockdown, Expressing, Derivative Assay

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on VEGFR2 protein expression in rat vascular endothelial cells. ( A ) Western blot bands of indicating proteins in endothelial cells; ( B ) densitometric analysis of the ratio of the intensity of protein bands. Values are the mean ± SD of VEGFR2 expression standardized to β-actin expression in three independent experiments; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on proliferation and migration of endothelial cells. ( A ) BrdU-positive cell rate; ( B ) migration distance statistics chart. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Migration

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on tube formation of endothelial cells. ( A ) Number of nodes forming a tube-like structure; ( B ) branch lengths forming the tube-like structure. Data expressed as mean ± SD; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the mouse matrigel plug model. ( A ) Pictures of the mouse matrigel plugs (a: PBS group, b: NC group, c: ROX group, d: sh-Vegfr2 group, and e: sh-Vegfr2 + ROX group); ( B ) hemoglobin content in matrigel plugs. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; # p < 0.05, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the growth and angiogenesis of/in melanoma xenografts. ( A ) Melanoma xenograft tumor volumes during the test period; ( B ) melanoma xenograft tumor weight at necropsy; ( C ) immunohistochemical expression of CD34 in melanoma xenografts. Data expressed as mean ± SD; * p < 0.05, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Expressing